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Easy and fast PCR-based protocol allows characterization of breakpoints resulting from Alu/Alu-mediated genomic

Filip Majer1, Jakub Sikora1,2

  • 1Research Unit for Rare Diseases, Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University, General University Hospital, Praha 2, Czech Republic.

Molecular Genetics & Genomic Medicine
|October 1, 2021
PubMed
Abstract

No abstract available in PubMed .

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...