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Updated: Oct 18, 2025

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Published on: December 6, 2017
Discovery of Antimicrobial Peptide Macrocycles Through Bacterial Display
Justin R Randall1, Cory D DuPai1,2, Bryan W Davies3
1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Abstract:
Peptide macrocycles exhibit great ability to inhibit bacterial growth making them a promising new avenue for antimicrobial discovery. Surface Localized Antimicrobial Display (SLAY) is a platform allowing the high-throughput screening of large peptide libraries of diverse length, composition, or structure for their antimicrobial activity, including macrocyclic peptides cyclized through disulfide bonding. Here we describe the procedure for the design and construction of a SLAY peptide library and the process for screening that library for antimicrobial potential.
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