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Related Concept Videos

Endoscopic Studies II: Thoracocentesis01:26

Endoscopic Studies II: Thoracocentesis

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Thoracentesis(Thoracocentesis), commonly known as pleural tap, is a medical procedure where a 22 gauge needle is inserted into the pleural space, the area between the lung and chest wall. This procedure is commonly performed to diagnose or treat various respiratory disorders.
Description
Excess pleural fluid or air may accumulate in some respiratory disorders in the thoracic cavity. To treat pleural effusion, a physician conducts thoracentesis by carefully piercing the chest wall and entering...
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Isolation of Tissue Extracellular Vesicles from the Liver
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Inducing, Collecting, and Storing Ascites.

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    This summary is machine-generated.

    Ascitic fluid, a high-antibody solution from mouse tumors, is collected for research. Adding sodium azide prevents bacterial and fungal contamination, ensuring antibody solution integrity.

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    Area of Science:

    • Immunology
    • Biotechnology
    • Animal Models

    Background:

    • Ascitic fluid is an intraperitoneal fluid collected from mice with induced peritoneal tumors.
    • Hybridoma cells are injected into the mouse peritoneum, serving as a bioreactor for antibody production.

    Purpose of the Study:

    • To describe the process and characteristics of ascitic fluid collection for antibody production.
    • To identify common storage issues and propose a preventative measure.

    Main Methods:

    • Induction of peritoneal tumors in mice using hybridoma cells.
    • Collection of ascitic fluid containing secreted antibodies.
    • Analysis of antibody concentration and storage stability.

    Main Results:

    • Ascitic fluid yields range from 1-10 mL per mouse.
    • Antibody concentrations typically range from 1-10 mg/mL.
    • Bacterial and fungal contamination is a common storage problem.

    Conclusions:

    • Ascitic fluid is a valuable source of high-titered antibodies.
    • Sodium azide addition is recommended to prevent contamination and preserve antibody solutions.