Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

15.3K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
15.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determining the Class and Subclass of Monoclonal Antibodies Using Antibody Capture on Anti-Immunoglobulin Antibody-Coated Plates.

Cold Spring Harbor protocols·2022
Same author

Immunizing Animals.

Cold Spring Harbor protocols·2022
Same author

Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Permeabilized Cells to Detect Intracellular Binding.

Cold Spring Harbor protocols·2022
Same author

Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Whole Cells to Detect Cell-Surface Binding.

Cold Spring Harbor protocols·2022
Same author

Hybridoma Screening by Antibody Capture: Enzyme-Linked Detection (Indirect ELISA) in Polyvinyl Chloride Wells.

Cold Spring Harbor protocols·2022
Same author

Determining the Class and Subclass of a Monoclonal Antibody by Ouchterlony Double-Diffusion Assays.

Cold Spring Harbor protocols·2022
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Sep 2, 2025

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

42.1K

Generating Monoclonal Antibodies.

Edward A Greenfield

    Cold Spring Harbor Protocols
    |August 1, 2022
    PubMed
    Summary
    This summary is machine-generated.

    This study details methods for creating and identifying monoclonal antibodies using hybridoma technology. These techniques are essential for producing specific antibody therapies and diagnostic tools.

    More Related Videos

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
    13:14

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

    Published on: February 6, 2018

    10.5K
    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
    10:32

    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

    Published on: June 5, 2015

    18.6K

    Related Experiment Videos

    Last Updated: Sep 2, 2025

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology
    09:42

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology

    Published on: January 2, 2017

    42.1K
    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
    13:14

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

    Published on: February 6, 2018

    10.5K
    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
    10:32

    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

    Published on: June 5, 2015

    18.6K

    Area of Science:

    • Biotechnology
    • Immunology

    Background:

    • Monoclonal antibodies are a critical class of proteins produced by hybridoma cell lines.
    • Hybridoma technology enables the production of highly specific antibodies for research and therapeutic applications.

    Purpose of the Study:

    • To present established methodologies for the generation and screening of monoclonal antibodies.
    • To outline the development of an effective screening procedure for identifying desired hybridomas.

    Main Methods:

    • Hybridoma cell line production through cell fusion.
    • Development and implementation of a robust antibody screening assay.
    • Characterization of antibody-producing hybridomas.

    Main Results:

    • Successful generation of hybridoma cell lines secreting specific monoclonal antibodies.
    • Validated screening procedures for efficient identification of high-affinity antibodies.
    • Established protocols for reproducible hybridoma production.

    Conclusions:

    • The described methods provide a reliable framework for monoclonal antibody discovery.
    • These techniques are fundamental for advancing antibody-based diagnostics and therapeutics.