Related Experiment Video

Updated: Sep 2, 2025

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

42.1K

Generating Monoclonal Antibodies

Edward A Greenfield

    Cold Spring Harbor Protocols
    |August 1, 2022
    PubMed

    Abstract:

    Antibodies that are produced by hybridomas are known as monoclonal antibodies. Here we introduce methods for generating and screening monoclonal antibodies, including developing the screening procedure and producing hybridomas.

    More Related Videos

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
    13:14

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

    Published on: February 6, 2018

    10.5K
    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
    10:32

    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

    Published on: June 5, 2015

    18.6K

    Related Experiment Videos

    Last Updated: Sep 2, 2025

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology
    09:42

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology

    Published on: January 2, 2017

    42.1K
    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
    13:14

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

    Published on: February 6, 2018

    10.5K
    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
    10:32

    Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

    Published on: June 5, 2015

    18.6K

    Related Concept Videos

    Hybridoma Technology01:31

    Hybridoma Technology

    15.3K
    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation,...
    15.3K

    Articles linked to this work by shared authors, journal, and citation graph.

    Determining the Class and Subclass of Monoclonal Antibodies Using Antibody Capture on Anti-Immunoglobulin Antibody-Coated Plates.

    Cold Spring Harbor protocols·2022

    Immunizing Animals.

    Cold Spring Harbor protocols·2022

    Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Permeabilized Cells to Detect Intracellular Binding.

    Cold Spring Harbor protocols·2022

    Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Whole Cells to Detect Cell-Surface Binding.

    Cold Spring Harbor protocols·2022

    Hybridoma Screening by Antibody Capture: Immunohistochemistry.

    Cold Spring Harbor protocols·2022

    Determining the Class and Subclass of Monoclonal Antibodies Using Antibody Capture on Antigen-Coated Plates.

    Cold Spring Harbor protocols·2022

    Procedures for Standardized Measurements of Core Traits in Maize Research and Breeding Programs.

    Cold Spring Harbor protocols·2026

    Standardized Trait Measurement in Maize Breeding and Genetics Research.

    Cold Spring Harbor protocols·2026

    Emerging Trends in Mass Spectrometry-Based Quantitative Proteome and Phosphoproteome Profiling in Maize.

    Cold Spring Harbor protocols·2026

    Sample Preparation for Quantitative Proteome and Phosphoproteome Profiling of Maize Tissues.

    Cold Spring Harbor protocols·2026

    High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic E. coli.

    Cold Spring Harbor protocols·2025

    Grain Quality in Maize.

    Cold Spring Harbor protocols·2025

    Safe Integration of Radiation Therapy With Bispecific Antibodies in Relapsed/Refractory B-cell Lymphomas.

    Clinical lymphoma, myeloma & leukemia·2026

    Dual delivery of immunostimulatory oligonucleotides and doxorubicin by magnetic nanoparticles and metal-organic frameworks for antitumor immunotherapy.

    Acta biomaterialia·2026

    Secretome of SHED Sheets Facilitates Mandibular Bone Defect Repair by Shifting Macrophages Inflammatory State.

    International dental journal·2026

    Bibliometric Analysis of Metabolic Reprogramming In T Lymphocytes Reveals Research Trends And Emerging Hotspots, 2016-2025.

    Journal of visualized experiments : JoVE·2026

    Time-Lapse Imaging of Antibody-Driven Macrophage Phagocytosis of Green Fluorescence Protein-Labeled Triple-Negative Breast Cancer Cells.

    Journal of visualized experiments : JoVE·2026

    Clinical characteristics of bloodstream infections in the patients receiving anti-IL-6 receptor antibody.

    Infection·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us