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Variability in the Processing of Fresh Osteochondral Allografts
Kyle D Paul1, Romil K Patel1, Alexandra M Arguello1
1Department of Orthopaedics, The University of Alabama at Birmingham, Birmingham, Alabama.
The Journal of Knee Surgery
|October 2, 2021
Summary
Fresh osteochondral allograft processing varies among tissue banks. Optimizing storage media, temperature, and growth factors like insulin growth factor-1 can improve chondrocyte viability and graft shelf-life.
Area of Science:
- Orthopedics
- Tissue Engineering
- Biotechnology
Background:
- Indications for fresh osteochondral allografts are rising, necessitating standardized processing protocols.
- Variations in tissue processing and preservation methods impact graft quality and clinical outcomes.
Approach:
- This study reviews and compares the literature on osteochondral allograft processing protocols from four leading US tissue banks.
- Key aspects examined include donor procurement, serological/microbiological testing, and storage procedures.
Key Points:
- Major tissue banks share similarities in donor screening, aseptic processing, and microbial testing.
- Significant variability exists in storage media, antibiotic use, storage temperature, and graft expiration dates.
Conclusions:
- Delicate tissue handling and optimized storage media can enhance chondrocyte viability.
- Incorporating growth factors like insulin growth factor-1 and storing grafts near physiologic temperatures may extend graft expiration time.
