Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combination of the First-in-Class Imipridone ONC201 and Standard Anticancer Therapies as a Rational Approach for Therapeutic Benefit.

Current issues in molecular biology·2025

Same author

ONC201 (Dordaviprone) Induces Integrated Stress Response and Death in Cervical Cancer Cells.

Biomolecules·2025

Same author

Dynamic Roles of Signaling Pathways in Maintaining Pluripotency of Mouse and Human Embryonic Stem Cells.

Cellular reprogramming·2024

Same author

At the Crossroads of TNF α Signaling and Cancer.

Current molecular pharmacology·2023

Same author

Molecular Milieu of Autophagy in Cervical Cancer and its Therapeutic Implications.

Current cancer drug targets·2023

Same author

The story of EGFR: from signaling pathways to a potent anticancer target.

Future medicinal chemistry·2022

Search research articles

Related Experiment Video

Updated: Oct 18, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

A flow cytometric journey into cell cycle analysis.

Anusree Nair¹, Sonal M Manohar¹

¹Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS (Deemed-to-be) University, Vile Parle (West), Mumbai, 400056, India.

|October 4, 2021

Summary

This summary is machine-generated.

Flow cytometry enables precise cell cycle analysis by measuring DNA content and molecular events. Recent advances enhance its utility for studying cellular responses and cell division dynamics.

Keywords:

DNA staining apoptosis cell cycle cell proliferation cell synchronization imaging flow cytometry

More Related Videos

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Related Experiment Videos

Last Updated: Oct 18, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Area of Science:

Cell Biology
Biotechnology
Analytical Chemistry

Background:

Cell cycle progression is fundamental to cell growth and division.
Understanding cell cycle dynamics is key to interpreting cellular responses to environmental stimuli.
Flow cytometry offers high-speed, single-cell resolution for biological analyses.

Purpose of the Study:

To review the evolution of flow cytometry techniques for cell cycle analysis.
To highlight the transition from DNA content measurement to multiparametric analysis.
To discuss recent advancements and their impact on cell cycle research.

Main Methods:

Review of established and novel flow cytometry protocols for cell cycle assessment.
Analysis of multiparametric data integrating DNA content with other cellular markers.
Examination of technological improvements in flow cytometry instrumentation and analysis.

Main Results:

Flow cytometry has evolved from DNA content analysis to comprehensive multiparametric cell cycle profiling.
Advanced protocols allow detailed investigation of molecular and cellular events during the cell cycle.
New techniques address limitations of traditional methods, broadening research scope.

Conclusions:

Flow cytometry is an indispensable tool for detailed cell cycle analysis.
Multiparametric approaches provide deeper insights into cell cycle regulation and response.
Ongoing technological advancements continue to expand the capabilities of flow cytometry in cell biology.