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Updated: Oct 17, 2025
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
Published on: May 15, 2020
Osteogenic Differentiation from Mouse Embryonic Stem Cells
Zahra Alvandi1,2, Michal Opas3
1Vascular Biology Program and Department of Surgery, Boston Children's Hospital, Boston, MA, USA.
Abstract:
Embryonic stem cells (ESCs) are a unique model that allows the study of molecular pathways underlying commitment and differentiation. We have studied signaling pathways and their contributions to osteogenic differentiation. In addition to our previously published protocol where we recommended the addition of retinoic acid with later addition of dexamethasone to boost osteogenic lineage cells, here we describe an optimized protocol for osteogenic differentiation from R1 ESCs with suggestions for inhibition of Src activity.
