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Comparative Glycomics Analysis of Mass Spectrometry Data
Yusen Zhou1, Sriram Neelamegham2
1Chemical and Biological Engineering, Biomedical Engineering and Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 6, 2021
Summary
This study introduces cGlyco, an open-source software for comparing glycan profiles across multiple mass spectrometry datasets. This tool aids in understanding functional glycomics and systems biology by analyzing complex carbohydrate structures.
Area of Science:
- Glycomics and Systems Biology
- Mass Spectrometry-based Glycan Analysis
Background:
- Glycan profiling is crucial for understanding cell and tissue carbohydrate structures.
- Functional glycomics and Systems Glycobiology rely on glycan distribution data.
- Comparing glycan profiles reveals differences in cell differentiation and epitopes.
Purpose of the Study:
- To develop and present cGlyco, an open-source program for comparative glycomics.
- To facilitate the comparison of data from multiple mass spectrometry runs.
- To aid in the analysis of functional glycomics data for systems biology applications.
Main Methods:
- Glycan release from cell/tissue samples.
- Functionalization of glycans (e.g., with 2-aminobenzamide) for enhanced LC/MS.
- Permethylation for improved glycan quantitation.
- Development and application of the cGlyco software for comparative analysis.
Main Results:
- Demonstration of cGlyco's utility in comparing multiple glycomics datasets.
- Application of cGlyco to MALDI-TOF glycomics profiling data.
- Successful comparison of glycan profiles from different samples.
Conclusions:
- cGlyco provides a valuable tool for comparative glycomics analysis.
- The software supports the understanding of functional glycomics and systems biology.
- Comparative glycan profiling enhances the study of cellular processes and differentiation.
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