Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Host plant nutrition drives fitness outcomes in the cactus specialist Drosophila mettleri.

PloS one·2026
Same author

Shielded by the dead: how killed bacteria shape the dynamics and evolution of innate immunity.

bioRxiv : the preprint server for biology·2026
Same author

Strong GAL4 expression compromises Drosophila fat body function.

Genetics·2025
Same author

Ovariole number does not predict reproductive output or trade-off with immunity in Drosophila melanogaster.

PloS one·2025
Same author

Strong GAL4 expression compromises <i>Drosophila</i> fat body function.

bioRxiv : the preprint server for biology·2025
Same author

Trade-off between antibacterial immune defense and oogenesis progression in female Drosophila melanogaster.

Genetics·2025

Related Experiment Video

Updated: Oct 17, 2025

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
12:48

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

Published on: March 25, 2014

15.0K

A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis.

Vanika Gupta1,2, Brian P Lazzaro1,2

  • 1Department of Entomology, Cornell University, Ithaca, NY, USA.

Fly
|October 6, 2021
PubMed
Summary

Researchers developed a new method to isolate single nuclei from the Drosophila fat body for RNA sequencing. This technique overcomes challenges with fragile cells, enabling better gene expression analysis in this vital insect tissue.

Keywords:
Fat bodyRNAseqimmune responsemetabolismprofilingscSeqsingle-cell sequencingtranscriptome

More Related Videos

Isolation of Drosophila melanogaster Testes
03:13

Isolation of Drosophila melanogaster Testes

Published on: May 13, 2011

32.7K
A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
06:54

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

Published on: September 5, 2019

7.2K

Related Experiment Videos

Last Updated: Oct 17, 2025

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
12:48

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

Published on: March 25, 2014

15.0K
Isolation of Drosophila melanogaster Testes
03:13

Isolation of Drosophila melanogaster Testes

Published on: May 13, 2011

32.7K
A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
06:54

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

Published on: September 5, 2019

7.2K

Area of Science:

  • Molecular Biology
  • Genomics
  • Developmental Biology

Background:

  • Traditional gene expression studies average cellular heterogeneity within tissues.
  • Single-cell RNA sequencing (sc-RNAseq) offers higher resolution but requires viable cell suspensions, which are difficult for some tissues.
  • The Drosophila fat body, crucial for physiological processes, comprises fragile cells challenging to dissociate for sc-RNAseq.

Purpose of the Study:

  • To develop a method for isolating single nuclei from the Drosophila adult fat body for RNA sequencing.
  • To enable transcriptomic analysis of individual cells within the fat body tissue.
  • To provide an alternative to cell dissociation methods that compromise cell viability.

Main Methods:

  • Developed a novel protocol for isolating single nuclei from the Drosophila adult fat body.
  • Utilized RNA sequencing on the isolated nuclei.
  • Compared the new method with existing nuclei preparation techniques.

Main Results:

  • The developed method effectively isolates single fat body nuclei.
  • The protocol minimizes mitochondrial contamination.
  • Achieved higher transcript capture per nucleus compared to other methods.
  • The method is suitable for single-cell nuclei sequencing and potentially bulk RNA sequencing.

Conclusions:

  • A robust method for isolating single nuclei from the Drosophila fat body has been established.
  • This technique facilitates high-resolution transcriptomic studies of the fat body.
  • The method offers a viable alternative for tissues with difficult-to-dissociate cells.