Localized surface plasmon resonance-based biosensor on gold nanoparticles for Taenia solium detection

This paper describes, for the first time to our knowledge, a fast-response and specific biosensor for detection of Taenia solium, a parasite responsible for neurocysticercosis disease that affects the central nervous system. The biosensor is based on the localized surface plasmon resonance (LSPR) technique on gold nanoparticles (AuNPs) in colloidal suspension that were functionalized and activated with antibodies to perform an immuno-capture effect. The AuNPs were synthetized by Turkevich and seed-mediated growth methods. A variety of concentrations of T. solium antigen were added to test the detection and the dose-response profile. Small antigen concentrations were detected indicating that the limit of detection is lower than 0.1 µg/mL of antigen. The results demonstrate the potential of the AuNPs LSPR biosensor as a clinical tool for neurocysticercosis diagnostic.