Graphene Nanoplatelets Render Poly(3-Hydroxybutyrate) a Suitable Scaffold to Promote Neuronal Network Development

Area of Science:

• Biomaterials Science
• Neuroscience
• Materials Engineering

Background:

• Composite biomaterials for neural interfaces are underdeveloped.
• Targeting neuro-pathologies requires advanced neural interface design.

Purpose of the Study:

• To evaluate poly(3-hydroxybutyrate)/graphene nanoplatelet (P(3HB)/GnP) composites as substrates for primary neurons.
• To explore the impact of GnP concentration on neuronal growth, network formation, and electrophysiological function.

Main Methods:

• Primary cortical neurons were cultured on P(3HB) scaffolds with varying GnP concentrations for 14 days.
• Biocompatibility, neuronal growth, and network maturation were assessed.
• Whole-cell patch-clamp recordings were used to investigate network functionality and neuronal excitability.

Main Results:

• All P(3HB)/GnP composite scaffolds were biocompatible, supporting physiological neuronal growth and network maturation.
• Pure P(3HB) reduced neuronal excitability, altering action potential waveforms and firing frequency.
• GnP incorporation restored electrophysiological parameters to physiological levels, with low concentrations promoting firing activity.

Conclusions:

• P(3HB)/GnP composites demonstrate excellent biocompatibility and promote neuronal network function.
• These composites show significant potential for creating effective neural interfaces for central nervous system disorders.
• Functionalized polymer scaffolds offer a promising strategy for advanced neuro-engineering applications.