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Updated: Oct 17, 2025

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
Published on: March 22, 2015
Yes, MAM!
Robert E Means1, Samuel G Katz1
1Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
Abstract:
Regulation of cell life and death by members of the BCL-2 family of proteins occurs at the mitochondria. Large portions of the mitochondria's outer membrane are found in tight approximation with the endoplasmic reticulum (ER), known as mitochondria-associated membranes (MAMs) or mitochondria-ER contact sites (MERCs). We recently reported that BOK is present within MAMs where it regulates Ca2+ transfer from the ER to the mitochondria, appropriate MAM components and MERC structure, and apoptosis.
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