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Related Experiment Video

Updated: Oct 17, 2025

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A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics.

Long Ma1, Lijuan Yin1, Xiaoyan Li2

  • 1State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China.

Biosensors & Bioelectronics
|October 8, 2021
PubMed
Summary
This summary is machine-generated.

A new CRISPR-Cas12a biosensor offers rapid, ultrasensitive detection of SARS-CoV-2, the virus causing COVID-19. This visual, smartphone-readable tool achieves single-copy resolution and shows high accuracy with clinical samples.

Keywords:
CRISPR-Cas12aClinical samplesGold nanoparticlesSARS-CoV-2 detectionSmartphone-based diagnosticsVisual biosensor

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Nanotechnology

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, necessitates advanced diagnostic tools.
  • Current detection methods require improvement in speed, sensitivity, and specificity.
  • CRISPR-Cas12a technology offers potential for precise nucleic acid detection.

Purpose of the Study:

  • To develop a rapid, sensitive, and selective visual biosensor for SARS-CoV-2 detection.
  • To integrate CRISPR-Cas12a with a smartphone readout for accessible diagnostics.
  • To validate the biosensor's performance using various sample types.

Main Methods:

  • Development of a CRISPR-Cas12a based visual biosensor.
  • Utilizing gold nanoparticles aggregation/disaggregation for colorimetric readout.
  • Smartphone application for quantitative analysis of color changes.
  • Testing with synthetic nucleic acids, pseudoviruses, and clinical samples.

Main Results:

  • The biosensor achieved ultrasensitive detection of SARS-CoV-2 with a limit of detection of 1 copy/μL for pseudoviruses.
  • Demonstrated high specificity with no cross-reactivity.
  • Achieved 100% agreement with qPCR results for clinical samples.
  • Provided results within approximately 90 minutes.

Conclusions:

  • The developed CRISPR-Cas12a visual biosensor is a robust technology for ultrasensitive SARS-CoV-2 detection.
  • The smartphone readout enables convenient and accessible diagnostics.
  • This method holds promise for clinical application in pandemic response.