Structure engineering of silicon nanoparticles with dual signals for hydrogen peroxide detection

Fluorescent silicon nanoparticles (SiNPs) were synthesized by a one-step, simple, and green method with 3-Aminopropyltriethoxysilane (APTES) and ascorbic acid (AA) as reaction agents. Subsequently, the SiNPs and AgNPs nanocomplex (SiNPs@AgNPs) was constructed as the probe for hydrogen peroxide (H₂O₂) detection. The fluorescence of SiNPs was quenched due to the surface plasmonic-enhanced energy transfer between SiNPs and AgNPs. Meanwhile, the color tends to be yellow due to the existence of AgNPs. As the AgNPs were etched by H₂O₂, the fluorescence recovers and color fadings. Based on the well-designed structure, the "off-on" fluorescence sensing and "on-off" color sensing platforms for H₂O₂ were fabricated. The as-synthesized materials were characterized by Fourier transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fluorescence and UV-vis absorption spectra were used to evaluate the optical performance. The fabricated sensor exhibited a linear range of 1.0-100.0 μM, with a limit of detection of 0.36 μM for the fluorescence sensing of H₂O₂. Additionally, a linear range of 1.0-50.0 μM and a limit of detection of 0.45 μM were displayed for the detection of H₂O₂ by colorimetric assay. The feasibility in complex medium of the fabricated fluorescent and colorimetric dual-signal sensor was evaluated by the detection of H₂O₂ in phosphate buffer saline (PBS) and lake water samples.