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[Determination of β-lactoglobulin in Anti-HPV Biological Protein Dressing by Amino Acid Assay]
Xiaomeng Zhang1, Jun Fu1, Kangjun Wang1
1Hainan Key Laboratory for Pharmaceutical Quality Research, Hainan Institute for Drug Control, Haikou, 570216.
Objective:
To establish an amino acid assay for the determination of β-lactoglobulin in Anti-HPV biological protein dressing.
Methods:
Under acidic conditions, β-lactoglobulin is hydrolyzed into free amino acids, separated by cation exchange chromatography, and derivatived after ninhydrin column. The chromatogram at 570 nm is collected. The content of β-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis.
Results:
β-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12 μg/mL (y=5.060x+4.278, r=0.999 7); The recovery rate (n=9) is 100.06%.
Conclusions:
The method is simple, specific, accurate and reproducible, which is suitable for the quantitative analysis of β-lactoglobulin in anti-HPV biological protein dressing.
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