Rational design of an HClO-specific triggered self-immolative fluorescent turn-on sensor and its bioimaging...

Area of Science:

• Analytical Chemistry
• Biomedical Sensing
• Fluorescence Spectroscopy

Background:

• Hypochlorous acid (HClO) is a crucial biomarker in disease pathogenesis.
• Developing rapid and selective detection methods for HClO is essential for early diagnosis and environmental monitoring.
• Existing detection methods may lack the speed, sensitivity, or visual accessibility required for real-time applications.

Purpose of the Study:

• To design and evaluate a novel HClO-specific fluorescent sensor (RESClO).
• To achieve rapid, selective, and sensitive detection of HClO in aqueous solutions.
• To demonstrate the sensor's utility in bioimaging and disease model visualization.

Main Methods:

• Synthesis of an N-protected Resorufin dye-based self-immolative fluorescent sensor (RESClO).
• Evaluation of sensor performance including response time, selectivity, and limit of detection (LOD).
• Application of the sensor for bioimaging in cells and zebrafish, and visual imaging of a mouse arthritis model.

Main Results:

• The RESClO sensor exhibits a rapid response to HClO within 10 seconds.
• High selectivity and sensitivity were achieved, with a limit of detection (LOD) of 16.8 nM.
• The sensor demonstrated successful bioimaging of HClO in cells and zebrafish, and visual imaging of arthritis models.

Conclusions:

• The RESClO sensor provides a simple, visual, and promising analytical tool for HClO detection.
• It offers potential for environmental monitoring and early diagnosis of HClO-related diseases.
• The sensor's performance in bioimaging and disease models highlights its practical applicability.