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Updated: Oct 17, 2025
Agarose Gel Electrophoresis for the Separation of DNA Fragments
Published on: April 20, 2012
Multiplasmid DNA in agarose gel electrophoresis: Suitable dye and temperature is essential for prominent profiles
Qiuxia Jiao1, Zhao Xin1, Qiufeng Gou1
1Department of Pathogenic Biology, Chengdu Medical College, Chengdu, P. R. China.
Abstract:
Nucleic acids dye Goldview is widely used in agarose gel electrophoresis (AGE). However, in this study, a sample of multiplasmid DNA (multi-pDNA) stained with Goldview analyzed by AGE showed its instability at low temperature. Three types of DNA samples were analyzed, including linear DNA (ladder), single-plasmid DNA (single-pDNA), and multi-pDNA, electrophoretic conditions were optimized by adjusting the dye, the buffer, and the temperature (1-50°C). The results showed that the light intensity of Gelred is 2.2-times higher than that of Goldview in staining multi-pDNA. Compared with the single-pDNA and the linear DNA, the multi-pDNA stained with Goldview was greatly affected by temperature. This short communication indicated that Gelred is a highly applicable dye for analyzing multiplasmid samples. The degree and the way of binding of Goldview to multi-pDNA are greatly affected by temperature.
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