Related Experiment Video
Updated: Oct 17, 2025
09:20
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
Published on: February 1, 2022
2.8K
Simultaneous profiling of multiple chromatin proteins in the same cells.
Sneha Gopalan1, Yuqing Wang2, Nicholas W Harper3
1Department of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Molecular Cell
|October 12, 2021
Summary
Multi-CUT&Tag allows simultaneous mapping of multiple proteins on chromatin within single cells. This epigenomic profiling method reveals protein co-localization and cell-type-specific chromatin architecture.
Area of Science:
- Epigenetics
- Molecular Biology
- Genomics
Background:
- Current chromatin mapping techniques like CUT&RUN and CUT&Tag analyze one protein at a time per cell.
- This limitation hinders the study of co-localization of different chromatin proteins and efficient use of limited samples.
Purpose of the Study:
- To develop a method for simultaneously mapping multiple proteins on chromatin in the same cell.
- To overcome the limitations of single-protein mapping approaches and increase information yield from single cells.
Main Methods:
- Adaptation of the CUT&Tag technique using antibody-specific barcodes.
- Simultaneous genome-wide mapping of multiple proteins, including histone modifications and RNA Polymerase II.
Main Results:
- Demonstrated highly specific multi-CUT&Tag maps revealing co-localization sites of chromatin proteins within single cells.
- Identified active and repressed genes, and candidate cis-regulatory elements.
- Enabled single-cell resolution for cell type identification and characterization of cell-type-specific chromatin architecture.
Conclusions:
- Multi-CUT&Tag significantly enhances the information content per cell in epigenomic mapping.
- Facilitates direct analysis of the interplay between different chromatin proteins in a single-cell context.
