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Updated: Oct 17, 2025
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
Published on: December 17, 2018
Rapid, ultrasensitive, highly selective detection of toxic Hg(II) ions in seabass, swordfish and water samples
Eda Erdemir1, Garen Suna2, Simay Gunduz2
1Organic Chemistry Laboratory, Chemistry Group, National Metrology Institute, (TUBITAK UME), 41470 Gebze, Kocaeli, Turkey; Faculty of Science, Department of Chemistry, Division of Inorganic Chemistry, Istanbul University, Istanbul, Turkey.
Abstract:
A novel fluorescent probe bearing triphenylamine, thiophene and thiosemicarbazide (TPA-TSC) was developed, and its distinct features were investigated. TPA-TSC demonstrated excellent selectivity towards Hg2+ ions, with a limit of detection (0.14 nM) among the lowest reported to date. It also exhibited a turn-off response by forming stable complex for a brief period (<30 s). Job plot analysis and high-resolution mass spectrometry (HRMS) revealed that TPA-TSC bound to Hg2+ with a 2:1 stoichiometry in an aqueous environment, remarkably the probe successfully detected Hg2+ ions in seabass, swordfish and different samples of water. In all, TPA-TSC offers a promising method for accurately determining the quality of seafood and water samples polluted with Hg2+.
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