Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.1K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.1K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.8K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phylloroseobilin Impairs Tumor Cell Migration and Vascular Network Stability via Cytoskeletal Destabilization.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Hydrophobic Refinement of Polarity-Switchable Lipo-Xenopeptides Modulates Endosomal Escape and Enhances mRNA Delivery In Vitro and In Vivo.

Journal of the American Chemical Society·2026
Same author

Endothelial Sprout Formation Is Regulated by Substrate Stiffness and Notch Signaling.

International journal of molecular sciences·2025
Same author

Homoharringtonine (omacetaxine mepesuccinate) limits the angiogenic capacity of endothelial cells and reorganises filamentous actin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Chemical Proteomics Reveals Human Off-Targets of Fluoroquinolone Induced Mitochondrial Toxicity.

Angewandte Chemie (International ed. in English)·2025
Same author

Design and Characterization of a Micro RNA-200c Detecting Broccoli Fluorescent Light-up Aptamer.

Chembiochem : a European journal of chemical biology·2025

Related Experiment Video

Updated: Oct 17, 2025

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

532

Mechanical Aspects of Angiogenesis.

Maibritt Kretschmer1, Daniel Rüdiger1, Stefan Zahler1

  • 1Department of Pharmacy, Pharmaceutical Biology, Ludwig-Maximilians-Universität München, Butenandtstraße 5-13, 81377 Munich, Germany.

Cancers
|October 13, 2021
PubMed
Summary

The extracellular matrix (ECM) stiffness and cell adhesion influence angiogenesis, impacting tissue regeneration and tumor growth. Understanding these mechanical cues offers new therapeutic strategies for tumor angiogenesis.

Keywords:
ECMangiogenesiscancer treatmentcell migrationendothelial cellsmechanical cuesstiffnesstip/stalk cellstumor angiogenesisvessel stabilization

More Related Videos

Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development
09:16

Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development

Published on: May 31, 2024

1.6K
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

14.1K

Related Experiment Videos

Last Updated: Oct 17, 2025

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

532
Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development
09:16

Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development

Published on: May 31, 2024

1.6K
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

14.1K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Oncology

Background:

  • Angiogenesis is vital for tissue regeneration and tumor growth.
  • While chemical signals like VEGF are known regulators, the role of the extracellular matrix (ECM) in angiogenesis is gaining importance.
  • Previous research focused on blood flow shear forces, overlooking direct ECM mechanical influences.

Purpose of the Study:

  • To investigate the influence of extracellular matrix (ECM) stiffness and cell adhesion on angiogenesis.
  • To highlight the mechanical cues governing key angiogenesis stages: cell migration, tip/stalk cell differentiation, and vessel stabilization.
  • To explore how ECM changes in tumors may dysregulate angiogenesis.

Main Methods:

  • Review and analysis of existing literature on mechanical factors in angiogenesis.
  • Focus on ECM stiffness and cell-ECM adhesion as key mechanical cues.
  • Examination of mechanical influences across distinct phases of angiogenesis.

Main Results:

  • ECM stiffness and cell adhesion significantly modulate endothelial cell behavior during angiogenesis.
  • Specific mechanical cues are required for different angiogenesis stages, including migration and stabilization.
  • Altered ECM properties during tumor growth can independently dysregulate angiogenesis.

Conclusions:

  • The mechanical properties of the ECM are critical regulators of angiogenesis, alongside biochemical signals.
  • Understanding ECM mechanics provides a basis for novel therapeutic strategies targeting tumor angiogenesis.
  • Modulating ECM stiffness presents a potential avenue for controlling pathological angiogenesis.