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Multienzyme nanoassemblies: from rational design to biomedical applications.

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Multienzyme nanoassemblies (MENAs) integrate multiple enzymes for enhanced performance. This review covers MENA design strategies, properties, and biomedical applications like biosensing and tumor therapy.

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Area of Science:

  • Biotechnology and Nanomedicine
  • Enzyme Engineering
  • Materials Science

Background:

  • Multienzyme nanoassemblies (MENAs) are gaining attention for combining multiple enzymes into a single entity.
  • This approach enhances enzymatic performance and opens potential for diverse applications.
  • Recent years have seen significant advancements in MENA design and fabrication.

Purpose of the Study:

  • To review current strategies for designing MENAs, focusing on substrate channeling, compartmentalization, and enzyme stoichiometry.
  • To discuss the properties of MENAs that enable important applications.
  • To summarize recent advances in biomedical applications of MENAs, including biosensing, tumor therapy, antioxidant applications, and drug delivery.

Main Methods:

  • Review of literature on MENA design strategies.
  • Analysis of desirable properties contributing to MENA functionality.
  • Summary of recent research on MENA applications in the biomedical field.

Main Results:

  • Key design strategies include substrate channeling, compartmentalization, and controlled enzyme stoichiometry.
  • MENAs exhibit desirable properties for various applications.
  • Significant progress has been made in utilizing MENAs for biosensing, tumor therapy, antioxidant functions, and drug delivery.

Conclusions:

  • MENAs offer improved enzymatic performance and broad application potential.
  • Further development is needed to overcome challenges and realize versatile MENA applications.
  • The review highlights current challenges and future perspectives in MENA development.