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Updated: Oct 16, 2025

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
Published on: January 1, 2017
Muscle repair after physiological damage relies on nuclear migration for cellular reconstruction
William Roman1,2, Helena Pinheiro2, Mafalda R Pimentel2
1Department of Experimental & Health Sciences, University Pompeu Fabra, CIBERNED, 08003 Barcelona, Spain.
Abstract:
Regeneration of skeletal muscle is a highly synchronized process that requires muscle stem cells (satellite cells). We found that localized injuries, as experienced through exercise, activate a myofiber self-repair mechanism that is independent of satellite cells in mice and humans. Mouse muscle injury triggers a signaling cascade involving calcium, Cdc42, and phosphokinase C that attracts myonuclei to the damaged site via microtubules and dynein. These nuclear movements accelerate sarcomere repair and locally deliver messenger RNA (mRNA) for cellular reconstruction. Myofiber self-repair is a cell-autonomous protective mechanism and represents an alternative model for understanding the restoration of muscle architecture in health and disease.
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