Machine learning modeling and analysis of biohydrogen production from wastewater by dark fermentation process

Dark fermentation process for simultaneous wastewater treatment and H₂ production is gaining attention. This study aimed to use machine learning (ML) procedures to model and analyze H₂ production from wastewater during dark fermentation. Different ML procedures were assessed based on the mean squared error (MSE) and determination coefficient (R²) to select the most robust models for modeling the process. The research showed that gradient boosting machine (GBM), support vector machine (SVM), random forest (RF) and AdaBoost were the most appropriate models, which were optimized by grid search and deeply analyzed by permutation variable importance (PVI) to identify the relative importance of process variables. All four models demonstrated promising performances in predicting H₂ production with high R² values (0.893, 0.885, 0.902 and 0.889) and small MSE values (0.015, 0.015, 0.016 and 0.015). Moreover, RF-PVI demonstrated that acetate, butyrate, acetate/butyrate, ethanol, Fe and Ni were of high importance in decreasing order.