Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nirmatrelvir-Ritonavir-Induced Pancreatitis: A Concern in the COVID Era.

ACG case reports journal·2026
Same author

Comment on 'Clinical impact of intratumoral HER2 heterogeneity on trastuzumab deruxtecan efficacy in patients with HER2-positive gastric cancer'.

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·2026
Same author

Enzymatic Synthesis of NAD<sup></sup>.

Current protocols·2026
Same author

Gasdermin-Mediated Pyroptosis: Novel Strategies Against Colorectal Cancer.

Cancer science·2026
Same author

Dual proximity-based interactome mapping of FKBP51 and FKBP52 uncovers shared metabolic networks.

Biochemical and biophysical research communications·2026
Same author

Facile Synthesis of H-L-Photo-Lysine and Its Genetic Incorporation Into Proteins.

Chembiochem : a European journal of chemical biology·2026
Same journal

Synthesis and biological evaluation of 6,9-disubstituted purine analogues inducing autophagy-mediated apoptosis and potentiating carboplatin response in high-grade serous ovarian cancer.

Bioorganic chemistry·2026
Same journal

Integrative multi-omics and machine learning reveal PLAUR as a Pan-Cancer prognostic biomarker and potential therapeutic target.

Bioorganic chemistry·2026
Same journal

Structure-guided modulation of the IL-4/IL-4Rα interface: molecular recognition, therapeutic antibodies, and small-molecule opportunities.

Bioorganic chemistry·2026
Same journal

Assessing the cytotoxic effects of group IX metal N-heterocyclic carbene complexes of iridium and rhodium on B16-F10 melanoma and non-cancerous RAW 264.7 cells.

Bioorganic chemistry·2026
Same journal

Structure-based discovery of a novel small-molecule inhibitor of ATG4B that modulates autophagy in esophageal squamous cell carcinoma therapy.

Bioorganic chemistry·2026
Same journal

Clinical drug-oriented design, synthesis, and pharmacological evaluation of novel dual-target antiepileptic hybrid molecules.

Bioorganic chemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 16, 2025

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Published on: April 9, 2021

5.5K

Profiling sirtuin activity using Copper-free click chemistry.

Alyson M Curry1, Ian Cohen2, Song Zheng2

  • 1Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23219, United States.

Bioorganic Chemistry
|October 16, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed novel azido-containing chemical probes to profile sirtuin activity. These probes exhibit good isoform selectivity, enabling the study of dynamic cellular protein activity changes in biological samples.

More Related Videos

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

2.1K
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.4K

Related Experiment Videos

Last Updated: Oct 16, 2025

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Published on: April 9, 2021

5.5K
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

2.1K
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.4K

Area of Science:

  • Biochemistry and Molecular Biology
  • Chemical Biology
  • Enzymology

Background:

  • Mammalian sirtuins are NAD+-dependent enzymes that remove acyl modifications from lysine residues.
  • Initially identified as histone deacetylases (HDACs), sirtuins target various cellular proteins, influencing gene expression, DNA repair, and metabolism.
  • Sirtuins are therapeutic targets for diseases like cancer and neurodegeneration, necessitating tools to study their activity.

Purpose of the Study:

  • To develop innovative chemical probes for profiling sirtuin activity in biological samples.
  • To create activity-based chemical probes (ABPs) for selective sirtuin detection and analysis.

Main Methods:

  • Synthesis of cyclooctyne-containing and azido-containing probes for copper-free click conjugation.
  • Evaluation of probe labeling efficiency and selectivity against recombinant sirtuins.
  • Analysis of azido-containing ABPs in protein mixtures and cell lysates for isoform-specific labeling.

Main Results:

  • Cyclooctyne-containing probes showed minimal labeling of recombinant sirtuins.
  • Azido-containing ABPs demonstrated good isoform selectivity for sirtuins.
  • Azido-containing ABPs successfully labeled individual sirtuin isoforms in complex biological samples.

Conclusions:

  • Azido-containing activity-based chemical probes are effective tools for studying sirtuin activity.
  • These biocompatible probes facilitate the investigation of dynamic changes in cellular protein activity.
  • The developed probes offer a promising approach for understanding sirtuin roles in health and disease.