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Impedance flow cytometry for viability analysis of Corynebacterium glutamicum
Fabian Stefan Franz Hartmann1, Ioannis Anastasiou1, Tamara Weiß1
1Department of Biotechnology and Biomedicine, Section for Synthetic Biology, Technical University of Denmark, Kongens, Lyngby, Denmark.
Journal of Microbiological Methods
|October 17, 2021
Abstract:
Corynebacterium glutamicum efficiently produces glutamate when growth is inhibited. Analyses of viability in this non-growing state requires time consuming plating and determination of colony forming units. We here establish impedance flow cytometry measurements to assess the viability of non-growing, glutamate producing C. glutamicum cultures within minutes.

