Related Experiment Video

Updated: Oct 16, 2025

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

112.5K

MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)

Guang-Lin Song1, Ming Xiao2, Xiao-Ya Wan1

  • 1Department of Oncology, People's Hospital of Yuechi County, Yuechi County, Sichuan Province, China.

Bioengineered
|October 18, 2021
PubMed

Insights

MicroRNA miR-130a-3p inhibits colorectal cancer (CRC) growth by targeting WNT1. This microRNA

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA miR-130a-3p exhibits anti-tumor properties across various cancers.
  • Wnt signaling is a key pathway implicated in tumorigenesis.
  • The specific roles of miR-130a-3p and Wnt1 pathway modulation in colorectal cancer (CRC) remain underexplored.

Purpose of the Study:

  • To investigate the function of miR-130a-3p in colorectal cancer.
  • To examine the contribution of Wnt1 pathway modulation by miR-130a-3p in CRC.

Main Methods:

  • Comparative analysis of miR-130a-3p expression in CRC tissues and cell lines versus normal controls.
  • Assessment of miR-130a-3p overexpression effects on CRC cell proliferation and WNT1 pathway factors (WNT1, c-myc, cyclin D1).
  • Dual-luciferase reporter assay to confirm WNT1 as a direct target of miR-130a-3p.

Main Results:

  • Downregulated miR-130a-3p was observed in CRC tissues and cell lines compared to normal controls.
  • Overexpression of miR-130a-3p significantly reduced CRC cell proliferation.
  • miR-130a-3p overexpression led to decreased expression of WNT1, c-myc, and cyclin D1; WNT1 was confirmed as a direct target, and its overexpression reversed miR-130a-3p's inhibitory effects.

Conclusions:

  • miR-130a-3p directly targets WNT1 to inhibit colorectal cancer growth.
  • miR-130a-3p and WNT1 pathway-associated factors represent potential therapeutic targets for CRC treatment.
Keywords:
Colorectal cancerWNT1Wnt signaling pathwaymiR-130a-3ptumor suppression

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
9.2K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.2K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.4K