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Fluorescent probes for visualizing ROS-associated proteins in disease
Hui Wang1, Xin Wang1, Ping Li1
1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Biomedical Sciences, Shandong Normal University Jinan 250014 P. R. China lip@sdnu.edu.cn tangb@sdnu.edu.cn.
Abstract:
Abnormal expression of proteins, including catalytic and expression dysfunction, is directly related to the development of various diseases in living organisms. Reactive oxygen species (ROS) could regulate protein expression by redox modification or cellular signal pathway and thus influence the development of disease. Determining the expression level and activity of these ROS-associated proteins is of considerable importance in early-stage disease diagnosis and the identification of new drug targets. Fluorescence imaging technology has emerged as a powerful tool for specific in situ imaging of target proteins by virtue of its non-invasiveness, high sensitivity and good spatiotemporal resolution. In this review, we summarize advances made in the past decade for the design of fluorescent probes that have contributed to tracking ROS-associated proteins in disease. We envision that this review will attract significant attention from a wide range of researchers in their utilization of fluorescent probes for in situ investigation of pathological processes synergistically regulated by both ROS and proteins.
Insights
Researchers are developing advanced fluorescent probes to track reactive oxygen species (ROS) and associated proteins, crucial for early disease diagnosis and new drug discovery.
Area of Science:
- Biochemistry
- Molecular Biology
- Medical Imaging
Background:
- Abnormal protein expression is linked to various diseases.
- Reactive oxygen species (ROS) influence disease development by regulating protein expression.
- Accurate detection of ROS-associated proteins is vital for disease diagnosis and drug development.
Purpose of the Study:
- To review recent advancements in fluorescent probes for tracking ROS-associated proteins.
- To highlight the role of these probes in understanding disease mechanisms.
- To provide insights for researchers in the field.
Main Methods:
- Review of literature on fluorescent probe design and application.
- Focus on probes for *in situ* imaging of ROS-related proteins.
- Analysis of probe performance in disease contexts.
Main Results:
- Significant progress in developing sensitive and specific fluorescent probes.
- Demonstration of probes' utility in visualizing ROS-protein interactions in diseases.
- Identification of key design principles for effective probes.
Conclusions:
- Fluorescence imaging offers a powerful, non-invasive method for studying ROS-protein dynamics.
- Developed fluorescent probes are instrumental in advancing *in situ* investigation of diseases.
- This field holds great promise for early disease detection and therapeutic target identification.
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