Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Absorption01:22

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Absorption

20
As individuals age, their body's physiology evolves, affecting drug pharmacokinetics. The most apparent changes occur in the gastrointestinal tract, where an increase in gastric pH, a delay in gastric emptying, and a reduction in gastrointestinal motility are observed. Remarkably, these changes do not substantially modify the absorption of orally administered drugs, particularly those absorbed via passive diffusion.Transdermal drug delivery emerges as a highly viable method for older adults due...
20
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

7.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
7.7K
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

2.9K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integration of the serum metabolome and gut microbiome underscores the importance of altered lipid metabolism and potential immune modulation in Parkinson's Disease.

Brain disorders (Amsterdam, Netherlands)·2026
Same author

Factors Associated With Food Allergy and Gut Microbiota Dysfunction.

JAMA pediatrics·2026
Same author

IBS stress reactivity phenotype is associated with blood transcriptome profiles and microstructural and functional brain changes.

Neurobiology of stress·2026
Same author

A microbiome quantitative trait locus in SLC39A8 modulates disease severity in synucleinopathy-induced models of Parkinson's disease.

Human molecular genetics·2026
Same author

Additional hospitalization costs associated with delirium among older adults: evidence from the Medicare Current Beneficiary Survey.

Frontiers in public health·2026
Same author

The Current Landscape of Microbial Biomarkers in Gastrointestinal Disease.

Gastroenterology clinics of North America·2026
Same journal

The Role of Nrf2 in SIRT1-Mediated RGC Neuroprotection in Traumatic Optic Neuropathy.

Translational vision science & technology·2026
Same journal

Explicit Inclusion of Diabetes Mellitus Without Retinopathy Within Diabetic Retinopathy Prediction.

Translational vision science & technology·2026
Same journal

Preclinical Safety and Feasibility Study of Line-Field Confocal Optical Coherence Tomography for Ophthalmology Applications.

Translational vision science & technology·2026
Same journal

Pathogenicity Analysis of Two Novel CRB1 Mutations in Three Chinese Inherited Retinal Dystrophy Families and a Literature Review.

Translational vision science & technology·2026
Same journal

Gas-Lesion Contact and Postural Compliance After Vitrectomy With Tamponade: A Continuous Monitoring and 3D Quantitative Analysis.

Translational vision science & technology·2026
Same journal

Automated Deep Learning Quantification of Avascular Area and Intravitreal Neovascularization in Retinal Flatmounts of Rodent Oxygen-Induced Retinopathy Models.

Translational vision science & technology·2026
See all related articles

Related Experiment Video

Updated: Oct 16, 2025

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

4.6K

The Ocular Microbiome Is Altered by Sampling Modality and Age.

William Katzka1,2, Tien S Dong1,2,3,4, Kayti Luu1,2,3,4

  • 1The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Translational Vision Science & Technology
|October 18, 2021
PubMed
Summary
This summary is machine-generated.

Choosing the right ocular sampling method is crucial for accurate microbiome analysis. Calcium alginate swabs best reflect corneal epithelial biopsies, while patient age also influences results.

More Related Videos

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.4K
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

3.7K

Related Experiment Videos

Last Updated: Oct 16, 2025

Conjunctival Commensal Isolation and Identification in Mice
07:52

Conjunctival Commensal Isolation and Identification in Mice

Published on: May 1, 2021

4.6K
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.4K
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

3.7K

Area of Science:

  • Ophthalmology
  • Microbiology
  • Genetics

Background:

  • Ocular microbiome studies lack standardized sampling methods.
  • No prior study directly compared different swabs to corneal epithelial biopsy.
  • This study addresses the need for comparative analysis of ocular sampling techniques.

Purpose of the Study:

  • To compare the accuracy of three conjunctival swab types against corneal epithelial biopsy.
  • To evaluate the impact of patient age on ocular microbiome composition.
  • To improve the reproducibility of ocular microbiome research.

Main Methods:

  • Compared calcium alginate swabs, cotton-tipped applicators, and Weck-Cel sponges to corneal epithelial biopsies.
  • Utilized 16S rRNA gene sequencing and real-time PCR for microbial analysis.
  • Analyzed concordance between swab types and biopsy, and assessed age-related microbiome differences.

Main Results:

  • Corneal biopsies revealed 31 genera, including Weisella, Corynebacterium, and Pseudomonas.
  • Calcium alginate swabs showed the highest concordance with corneal biopsies.
  • Cotton swabs missed certain bacterial populations, and Weck-Cel swabs differed significantly.
  • Older patients (≥65) exhibited higher ocular microbiome alpha diversity.

Conclusions:

  • Ocular sampling method significantly impacts sequencing-based microbiome analysis outcomes.
  • Patient age is another critical factor influencing ocular microbiome diversity and composition.
  • Standardizing sampling techniques is essential for reliable ocular microbiome research.