Related Experiment Video
Updated: Oct 16, 2025
Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
Published on: May 31, 2024
Mandibular bone regeneration with autologous adipose-derived mesenchymal stem cells and coralline hydroxyapatite:
F J Barrientos-Lezcano1, L M Redondo-González2, M Alberca-Zeballos3
1Oral and Maxillofacial Surgeon, Servicio de Cirugía Maxilofacial, Hospital General Universitario de Castellón, Avenida de Benicassim 128, 12004, Castellón, Spain.
Abstract:
The purpose of this paper was to investigate the bone regeneration effect of autologous adipose tissue mesenchymal stem cells (ATMSC) in a small animal model. Twelve Wistar rats were given bilateral critical-size defects in the mandible. The defects were filled with coralline hydroxyapatite alone or combined with autologous undifferentiated ATMSC obtained from the dorsal fat pad. Studies were conducted at three and six weeks. Descriptive histology and histomorphometry revealed a significant (p < 0.05) increased bone regeneration values in the cell-treated defects at both three and six weeks. ATMSC promoted the formation of new bone in the central areas of the defects and in the scaffold micropores, both in a higher state of maturation. Autologous undifferentiated ATMSC enhanced bony healing of mandibular critical-size defects in rats when implanted with a coralline hydroxyapatite scaffold.
