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Related Experiment Video

Updated: Oct 16, 2025

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
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Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

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Xenopus Explants and Transplants.

Sally A Moody1

  • 1Department of Anatomy and Cell Biology, The George Washington University, School of Medicine and Health Sciences, Washington, D.C. 20037, USA samoody@gwu.edu.

Cold Spring Harbor Protocols
|October 20, 2021
PubMed
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This study details embryological manipulation techniques in Xenopus, enabling analysis of tissue inductive signals, cell migration, and organogenesis. These methods are easily applicable to both Xenopus laevis and Xenopus tropicalis embryos.

Area of Science:

  • Developmental biology
  • Embryology
  • Xenopus research

Background:

  • Traditional methods assess embryonic potential via tissue transplantation or explant culture.
  • Understanding inductive signals, cell migration, and organogenesis is crucial in developmental studies.

Purpose of the Study:

  • To introduce practical embryological manipulation techniques for Xenopus.
  • To facilitate the analysis of key developmental processes like induction, migration, and organogenesis.

Main Methods:

  • Describes several types of embryological manipulations in Xenopus embryos.
  • Techniques are based on classical, easily performable approaches.

Main Results:

  • The introduced protocols are particularly useful for analyzing tissue inductive signals.

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Last Updated: Oct 16, 2025

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
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Published on: February 2, 2016

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Neural Explant Cultures from Xenopus laevis

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  • These methods aid in studying cell migration and organogenesis.
  • The techniques are applicable to both Xenopus laevis and Xenopus tropicalis.

    • Conclusions:

    • The described Xenopus manipulation techniques offer a valuable toolkit for developmental biologists.
    • These methods provide a straightforward way to investigate fundamental embryonic processes.