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Related Experiment Video

Updated: Oct 16, 2025

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

Frank Klont¹, Marcel Kwiatkowski^1,2, Alen Faiz³

¹Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.

Journal of Proteome Research

|October 20, 2021

Summary

This summary is machine-generated.

Adsorptive microtiter plates can now be used for protein enrichment before liquid chromatography-mass spectrometry (LC-MS) analysis. This method enhances protein purification workflows for both targeted and discovery-based mass spectrometry research.

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Published on: August 16, 2019

Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Affinity ligands like antibodies are crucial for purifying proteins from biological samples.
Solid supports immobilize ligands, aiding protein separation.
Adsorptive microtiter plates are common for immunoassays but underutilized for LC-MS.

Purpose of the Study:

To demonstrate the utility of adsorptive microtiter plates for protein enrichment prior to LC-MS detection.
To explore the opportunities and challenges of using these plates in LC-MS workflows.
To highlight the potential of adsorptive plates in mass spectrometry-based protein research.

Main Methods:

Utilized adsorptive microtiter plates for protein enrichment.
Applied the method to targeted LC-MS analysis of soluble receptor for advanced glycation end-products (sRAGE).
Applied the method to discovery-based LC-MS analysis of transcription factor p65 (NF-κB) and FKBP5.

Main Results:

Successfully employed adsorptive microtiter plates for protein enrichment in both targeted and discovery-based LC-MS workflows.
Demonstrated applicability across different biological samples (serum, cell lysates).
Provided examples for sRAGE, NF-κB, and FKBP5 enrichment.

Conclusions:

Adsorptive microtiter plates offer a viable approach for protein enrichment before LC-MS analysis.
This technique can enhance existing affinity purification workflows.
Increased adoption of adsorptive plates could benefit mass spectrometry-based protein research.