Micronucleus test and nuclear abnormality assay in zebrafish (Danio rerio): Past, present, and future trends

Nuclear abnormality (NA) assay in fish has been widely applied for toxicity risk assessment under field and laboratory conditions. The zebrafish (Danio rerio) has become a suitable model system for assessing the NA induced by pollutants. Thus, the current study aimed to summarize and discuss the literature concerning micronucleus (MN) and other NA in zebrafish and its applications in toxicity screening and environmental risk assessment. The data concerning the publication year, pollutant type, experimental design, and type of NA induced by pollutants were summarized. Also, molecular mechanisms that cause NA in zebrafish were discussed. Revised data showed that the MN test in zebrafish has been applied since 1996. The MN was the most frequently NA, but 15 other nuclear alterations were reported in zebrafish, such as notched nuclei, blebbed nuclei, binucleated cell, buds, lobed nuclei, bridges, and kidney-shaped. Several pollutants can induce NA in zebrafish, mainly effluents (mixture of pollutants), agrochemicals, and microplastics. The pollutant-induced NA in zebrafish depends on experimental design (i.e., exposure time, concentration, and exposure condition), developmental stages, cell/tissue type, and the type of pollutant. Besides, research gaps and recommendations for future studies are indicated. Overall, the current study showed that zebrafish is a suitable model to assess pollutant-induced mutagenicity.