Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

322
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
322
DNA Base Pairing02:27

DNA Base Pairing

29.9K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
29.9K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.9K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.9K
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

4.8K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
4.8K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.7K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.7K
Genome Copying Errors02:46

Genome Copying Errors

4.6K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A role of transcription factors in pathogenic processes associated with schizophrenia].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2024
Same author

Histone Tetrasome Dynamics Affects Chromatin Transcription.

bioRxiv : the preprint server for biology·2024
Same author

[The Role of the WGR Domain in the Functions of PARP1 and PARP2].

Molekuliarnaia biologiia·2023
Same author

Poly(ADP-Ribosyl) Code Functions.

Acta naturae·2021
Same author

Histone Tails Promote PARP1-Dependent Structural Rearrangements in Nucleosomes.

Doklady. Biochemistry and biophysics·2020
Same author

[Opposite Effects of Histone H1 and HMGN5 Protein on Distant Interactions in Chromatin].

Molekuliarnaia biologiia·2019

Related Experiment Video

Updated: Oct 16, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

898

[Guanine Quadruplexes in Cell Nucleus Metabolism].

E V Marilovtseva1,2,3, V M Studitsky1,4

  • 1Bioengineering Department, Faculty of Biology, Moscow State University, Moscow, 119234 Russia.

Molekuliarnaia Biologiia
|October 21, 2021
PubMed
Summary

Guanine quadruplexes (G4) are crucial nucleic acid structures regulating gene expression and cellular processes. Their unique structure, stability, and interactions with proteins make them promising targets for anticancer therapies.

Keywords:
DNAG4RNAguanine quadruplexesnucleic acid secondary structureregulation of gene expression

More Related Videos

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.5K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.2K

Related Experiment Videos

Last Updated: Oct 16, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

898
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.5K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.2K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Cell metabolism relies on precise gene expression regulation.
  • Nucleic acid secondary structures, particularly guanine quadruplexes (G4), are key regulatory elements.
  • G4 structures are dynamic and influenced by ionic conditions, size, and nucleic acid composition.

Purpose of the Study:

  • To review the structure and thermodynamic properties of G-quadruplexes.
  • To explore the interactions between G-quadruplexes and nuclear proteins.
  • To highlight the significance of G4 structures in biological processes and as therapeutic targets.

Main Methods:

  • Literature review focusing on G-quadruplex structure and thermodynamics.
  • Analysis of G-quadruplex interactions with nuclear proteins.
  • Synthesis of current research on G4 roles in gene regulation and disease.

Main Results:

  • G-quadruplex stability is modulated by various factors including ion concentration and sequence.
  • G4 structures are involved in critical cellular functions like telomere maintenance and translation initiation.
  • Specific nuclear proteins interact with G4s, influencing their formation and function.

Conclusions:

  • G-quadruplexes are vital regulators of gene expression with diverse biological roles.
  • Understanding G4 structure-thermodynamics and protein interactions is crucial for biological insights.
  • G4s represent a promising frontier for novel anticancer drug development.