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Updated: Oct 16, 2025

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
Published on: July 6, 2017
Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins
Sally Adams1, Isabelle A Carré2
1School of Life Sciences, University of Warwick, Coventry, UK.
Abstract:
Chromatin immunoprecipitation, or ChIP, is a powerful experimental technique for probing protein-DNA interactions in vivo. This assay can be used to investigate the association of a protein of interest with specific target loci. Alternatively, it can be combined with high-throughput sequencing technology to identify genome-wide binding sites. Here, we describe a ChIP protocol that was optimized for low-abundance transcription factors in Arabidopsis, and provide guidance on how to adapt it for other types of plants and proteins.
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