Related Experiment Video
Updated: Oct 15, 2025
07:40
Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
Published on: June 2, 2017
15.3K
Metal Nanoparticles in Laser Bioprinting.
Vyacheslav Zhigarkov1, Ivan Volchkov1, Vladimir Yusupov1
1Federal Scientific Research Centre "Crystallography and Photonics", Russian Academy of Sciences, Pionerskaya St., 2, 108840 Moscow, Russia.
Nanomaterials (Basel, Switzerland)
|October 23, 2021
Summary
Laser bioprinting uses a laser to transfer cell-laden hydrogel droplets. This study quantizes titanium nanoparticles transferred during laser bioprinting, finding minimal amounts that do not harm cells.
Area of Science:
- Biotechnology
- Tissue Engineering
- Regenerative Medicine
Background:
- Laser bioprinting transfers cell-laden hydrogel droplets using laser-induced microdroplet ejection.
- Metal absorption layers used in laser bioprinting can generate nanoparticles, potentially impacting bioink quality and cell viability.
Purpose of the Study:
- To experimentally quantify the amount of titanium (Ti) nanoparticles formed and transferred during laser bioprinting.
- To investigate the influence of laser pulse fluence on microdroplet volume and nanoparticle transfer.
Main Methods:
- Investigated Ti films (25-400 nm thickness) produced by magnetron sputtering as absorption layers.
- Performed experimental measurements of nanoparticle formation and transfer in bioink microdroplets under varying laser fluences.
Main Results:
- Quantified the volume of bioink microdroplets and the concentration of Ti nanoparticles within them.
- Found that 0.5% to 2.5% of nanoparticles are transferred with the bioink microdroplets, while the majority remain on the donor slide.
Conclusions:
- The amount of transferred Ti nanoparticles during laser bioprinting is minimal.
- These low levels of nanoparticles are unlikely to negatively affect the viability or function of printed cells in biotechnological applications.
