Metal Nanoparticles in Laser Bioprinting

Laser bioprinting is a promising method for applications in biotechnology, tissue engineering, and regenerative medicine. It is based on a microdroplet transfer from a donor slide induced by laser pulse heating of a thin metal absorption film covered with a layer of hydrogel containing living cells (bioink). Due to the presence of the metal absorption layer, some debris in the form of metal nanoparticles is printed together with bioink microdroplets. In this article, experimental investigations of the amount of metal nanoparticles formed during the laser bioprinting process and transported in bioink microdroplets are performed. As metal absorption layers, Ti films with the thickness in the range of 25-400 nm, produced by magnetron spattering, were applied. Dependences of the volume of bioink microdroplets and the amount of Ti nanoparticles within them on the laser pulse fluence were obtained. It has been experimentally found that practically all nanoparticles remain in the hydrogel layer on the donor slide during bioprinting, with only a small fraction of them transferred within the microdroplet (0.5% to 2.5%). These results are very important for applications of laser bioprinting since the transferred metal nanoparticles can potentially affect living systems. The good news is that the amount of such nanoparticles is very low to produce any negative effect on the printed cells.