Related Experiment Video
Updated: Oct 15, 2025
Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
Published on: May 2, 2016
Characterization of Gold Nanorods Conjugated with Synthetic Glycopolymers Using an Analytical Approach Based on
Milica Velimirovic1,2, Alessia Pancaro2,3, Robert Mildner4
1Department of Chemistry, Atomic & Mass Spectrometry-A&MS Research Group, Campus Sterre, Ghent University, Krijgslaan 281-S12, 9000 Ghent, Belgium.
Abstract:
A new comprehensive analytical approach based on single-particle inductively coupled plasma-sector field mass spectrometry (spICP-SFMS) and electrical asymmetric-flow field-flow-fractionation combined with multi-angle light scattering detection (EAF4-MALS) has been examined for the characterization of galactosamine-terminated poly(N-hydroxyethyl acrylamide)-coated gold nanorods (GNRs) in two different degrees of polymerization (DP) by tuning the feed ratio (short: DP 35; long: DP 60). spICP-SFMS provided information on the particle number concentration, size and size distribution of the GNRs, and was found to be useful as an orthogonal method for fast characterization of GNRs. Glycoconjugated GNRs were separated and characterized via EAF4-MALS in terms of their size and charge and compared to the bare GNRs. In contrast to spICP-SFMS, EAF4-MALS was also able of providing an estimate of the thickness of the glycopolymer coating on the GNRs surface.
