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Related Experiment Video

Updated: Oct 15, 2025

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling.

Yanwen Ye^1,2, Zefeng Wang^1,2, Yun Yang¹

¹Bio-med Big Data Center, CAS Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, China.

|October 25, 2021

Summary

This summary is machine-generated.

This study introduces a sensitive new method to identify translatable circular RNAs (circRNAs). The technique uses polysome profiling and circRNA-seq, improving upon less sensitive ribosome footprinting and proteomic analyses.

Keywords:

Polysome profiling RNA-seq RNase R back-splicing junction circRNA translation

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Last Updated: Oct 15, 2025

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

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Published on: October 28, 2014

An Easy Method for Plant Polysome Profiling

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Published on: August 28, 2016

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

Area of Science:

Molecular Biology
Genomics
RNA Biology

Background:

Circular RNAs (circRNAs) are eukaryotic RNAs regulating gene expression, often acting as miRNA sponges.
Exonic circRNAs can be translated via cap-independent mechanisms (IRESs, m6A methylation).
The full range of translatable circRNAs and their functions remain largely unknown.

Purpose of the Study:

To develop a more sensitive method for identifying translatable circRNAs.
To overcome limitations of existing ribosome footprinting and proteomic analyses.

Main Methods:

Utilized polysome profiling to isolate actively translated RNAs.
Enriched circRNAs from polysome fractions using RNase R treatment.
Identified circRNAs via paired-end deep sequencing (circRNA-seq).

Main Results:

The described method demonstrates higher sensitivity for detecting translatable circRNAs.
Successfully identified translatable circRNAs using polysome profiling and circRNA-seq.
This approach improves upon the efficiency of discovering circRNA-specific reads or peptides.

Conclusions:

Polysome profiling combined with circRNA-seq offers a sensitive alternative for identifying translatable circRNAs.
This method enhances the scope of circRNA translation research.
Further investigation into the biological roles of translated circRNAs is warranted.