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Modulating OPG and TGF-β1 mRNA expression via bioelectrical stimulation.

Sejal D Chaudhari1, Kapil K Sharma1, John J Marchetto1,2

  • 1OrthodontiCell Inc., Leonhardt Launchpads Utah, Inc., Salt Lake City, UT 84115, United States of America.

Bone Reports
|October 25, 2021
PubMed
Summary
This summary is machine-generated.

Bioelectric stimulation (BES) noninvasively modulates osteoprotegerin (OPG) and transforming growth factor β1 (TGF-β1) to enhance bone remineralization. This novel approach accelerates tooth anchorage and reduces post-treatment complications in orthodontics.

Keywords:
Bone remodeling/regenerationElectrophysiologyGene expressionGrowth factorMineralization in vitroOrthodontic tooth movementOsteoblast

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Orthodontics

Background:

  • Bone remodeling is crucial for dental and skeletal health, involving osteoblasts secreting OPG and TGF-β1.
  • Orthodontic tooth movement requires stabilization, with current methods like OPG injections having limitations.
  • Noninvasive bioelectric stimulation (BES) offers a novel approach to modulate bone remodeling factors.

Purpose of the Study:

  • To investigate how various BES parameters influence OPG and TGF-β1 expression in osteoblasts.
  • To explore BES as a noninvasive method for enhancing bone remineralization.

Main Methods:

  • Osteoblasts were cultured and exposed to a range of frequencies (25 Hz to 3 MHz).
  • Quantitative real-time PCR (RT-qPCR) was used to measure OPG and TGF-β1 mRNA expression.
  • Alizarin Red staining assessed calcium deposition to evaluate mineralization.

Main Results:

  • BES significantly upregulated OPG mRNA expression above 10 kHz, peaking at 100 kHz (332% increase).
  • TGF-β1 mRNA expression increased across all tested frequencies, peaking at 250 kHz (332% increase).
  • In vitro mineralization increased by 28% after 6 weeks of stimulation.

Conclusions:

  • BES effectively modulates OPG and TGF-β1 mRNA expression in osteoblasts.
  • Noninvasive BES shows potential for accelerating bone remineralization and improving orthodontic anchorage.
  • BES may offer a new therapeutic tool for bone remodeling in various clinical applications.