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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

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Analysis of mRNA Translation by Polysome Profiling

Anne Cammas1,2, Pauline Herviou1,2, Leïla Dumas1,2

  • 1Cancer Research Center of Toulouse, INSERM UMR 1037, Toulouse, France.

Methods in Molecular Biology (Clifton, N.J.)
|October 25, 2021
PubMed

Insights

This study details polysome profiling, a method to analyze mRNA translation and associated proteins. It helps understand how cells regulate protein production during disease.

Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • Cellular Signaling

Background:

  • mRNA translation is crucial for cellular proteome modulation in response to signals.
  • Polysome profiling offers comprehensive analysis of mRNA translation states and associated proteins.
  • Understanding translation regulation is vital in pathophysiological contexts.

Purpose of the Study:

  • To describe a laboratory procedure for polysome profiling.
  • To investigate molecular mechanisms of translation regulation.
  • To study translation under pathophysiological conditions.

Main Methods:

  • Detailed description of the polysome profiling technique.
  • Analysis of mRNA and protein complexes.
  • Application in disease models.

Main Results:

  • Provides a robust protocol for polysome profiling.
  • Enables detailed examination of translation regulation.
  • Facilitates insights into disease-related molecular mechanisms.

Conclusions:

  • Polysome profiling is an effective method for studying translation regulation.
  • The described procedure aids research into disease mechanisms.
  • This technique is valuable for understanding cellular responses to stress.
Keywords:
Polysome profilingRibosomeTranslatomemRNA translation

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