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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

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Published on: September 16, 2019

Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq

Yinzhou Zhu¹, Christopher L Holley^1,2, Gordon G Carmichael³

¹Department of Medicine (Cardiology Division), Duke University School of Medicine, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 25, 2021

Abstract:

The ability to detect 2'-O-methylation sites (Nm) in high-throughput fashion is important, as increasing evidence points to a more diverse landscape for this RNA modification as well as the possibility of yet unidentified functions. Here we describe an optimized version of RibOxi-seq, which is built upon the original published method, that not only accurately profiles ribosomal RNA (rRNA) Nm sites with minimal RNA input but is also robust enough to identify mRNA intronic and exonic sites.

Keywords:

2′-O-methylation Nm RNA modifications Ribose methylation mRNA modifications rRNA modifications

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