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Updated: Oct 15, 2025

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Microfluidic Biosensor for Rapid Nucleic Acid Quantitation Based on Hyperspectral Interferometric Amplicon-Complex

Rongxin Fu1, Wenli Du1, Xiangyu Jin1

  • 1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.

ACS Sensors
|October 25, 2021
PubMed
Summary
This summary is machine-generated.

A new hyperspectral interferometric method enables rapid, quantitative nucleic acid detection using recombinase polymerase amplification (RPA). This technique significantly reduces reaction time and avoids false positives, making nucleic acid analysis more accessible.

Keywords:
hyperspectral interferometrymalaria detectionmicrofluidic biosensoroptical scattering analysisrecombinase polymerase amplification

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Area of Science:

  • Biomedical engineering
  • Molecular biology
  • Analytical chemistry

Background:

  • Nucleic acid detection is crucial in medicine and research.
  • Traditional polymerase chain reaction (PCR) is slow and complex.
  • Current recombinase polymerase amplification (RPA) detection methods are costly, complex, and prone to false positives, hindering widespread adoption.

Purpose of the Study:

  • To develop a novel, rapid, and cost-effective method for nucleic acid detection using RPA.
  • To overcome the limitations of existing RPA amplicon detection techniques, including complexity, cost, and false positives.
  • To create an integrated, automated system for sensitive and quantitative nucleic acid analysis.

Main Methods:

  • A hyperspectral interferometric amplicon-complex quantitation method was developed.
  • Asymmetric dipole complex strategy optical scattering analysis was employed.
  • GelRed dye formed amplicon-complex particles, with Fourier domain spectrum computation for scattering quantitation.
  • A microfluidic chip and automated system were integrated for sample processing and detection.

Main Results:

  • The method achieved integrated, rapid (20 min), quantitative, and sensitive nucleic acid detection.
  • The limit of detection was 3.17 ng/μL, with a minimum measurable concentration of 1.68 copies/μL (31.67 times more sensitive than turbidity).
  • The system successfully detected single-nucleotide polymorphisms in the *Plasmodium falciparum dhfr* gene and avoided false positives.

Conclusions:

  • The developed hyperspectral interferometric method offers a rapid, automated, and low-cost solution for nucleic acid detection.
  • This technique enhances the sensitivity and reliability of RPA-based diagnostics.
  • The integrated system shows significant potential for advancing point-of-care nucleic acid testing and genetic analysis.