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Testing the effects of processing on donor human Milk: Analytical methods
Eva Kontopodi1, Kasper Hettinga2, Bernd Stahl3
1Amsterdam UMC, University of Amsterdam, Vrije Universiteit, Emma Children's Hospital, Human Milk Bank, Amsterdam, the Netherlands; Food Quality and Design Group, Wageningen University & Research, the Netherlands.
Food Chemistry
|October 26, 2021
Summary
Holder pasteurization of donor milk kills microbes but reduces beneficial properties. This study reviews analytical methods to evaluate new treatments that better preserve milk
Area of Science:
- Food Science
- Biotechnology
- Analytical Chemistry
Background:
- Holder pasteurization is standard for donor human milk (DHM) safety.
- This thermal process compromises DHM's nutritional and bioactive components.
- Novel processing methods are needed to balance safety and functionality.
Purpose of the Study:
- To provide a comprehensive overview of analytical techniques for evaluating DHM processing methods.
- To propose an efficient analytical workflow for processed DHM.
- To guide the selection of methods that preserve DHM's functional properties.
Main Methods:
- Review of analytical techniques for microbial inactivation, nutritional content, and bioactivity.
- Discussion of safety and functionality analytical panels.
- Evaluation of technique principles, benefits, and drawbacks.
Main Results:
- Identified key analytical targets for safety and functionality assessment.
- Highlighted the strengths and limitations of various analytical assays.
- Emphasized the need for complementary assays for cross-validation.
Conclusions:
- Evaluating novel DHM processing requires a multifactorial approach.
- Combining safety and functionality analyses is crucial.
- Cross-validation using complementary assays ensures reliable results.
