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Silencing of LncRNA AFAP1-AS1 Inhibits Cell Proliferation in Oral Squamous Cancer by Suppressing CCNA2

Tao Li1, Duanqin Liu1, Chenglong Li1

  • 1Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, People's Republic of China.

Cancer Management and Research
|October 27, 2021
PubMed
Abstract

Insights

Long noncoding RNA AFAP1-AS1 is upregulated in oral squamous cell carcinoma (OSCC), promoting tumor growth and metastasis. Targeting AFAP1-AS1 may offer a new therapeutic strategy for OSCC patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various diseases, including cancer.
  • The lncRNA AFAP1-AS1 plays a role in oncogenesis, metastasis, and drug resistance across several cancer types.
  • The specific function of AFAP1-AS1 in oral squamous cell carcinoma (OSCC) remains largely uncharacterized.

Purpose of the Study:

  • To investigate the role and mechanism of lncRNA AFAP1-AS1 in oral squamous cell carcinoma (OSCC).
  • To determine the clinical significance of AFAP1-AS1 expression in OSCC progression.

Main Methods:

  • Bioinformatics analysis of AFAP1-AS1 expression in OSCC tissues versus normal controls.
  • Loss-of-function assays to assess the biological impact of AFAP1-AS1.
  • High-throughput sequencing and bioinformatics for mechanistic studies.
  • In vivo xenograft assays to evaluate AFAP1-AS1's effect on tumor growth.

Main Results:

  • AFAP1-AS1 expression is significantly upregulated in OSCC.
  • Elevated AFAP1-AS1 levels correlate with lymph node metastasis, advanced tumor stage, and higher pathological grade.
  • Downregulation of AFAP1-AS1 inhibits OSCC cell proliferation in vitro and tumor growth in vivo.
  • AFAP1-AS1 promotes OSCC proliferation by targeting CCNA2.

Conclusions:

  • AFAP1-AS1 acts as an oncogenic lncRNA in OSCC, promoting tumor proliferation.
  • AFAP1-AS1 expression serves as a prognostic biomarker for poor outcomes in OSCC.
  • Targeting AFAP1-AS1 presents a potential therapeutic avenue for OSCC treatment.
Keywords:
AFAP1-AS1CCNA2LncRNAbioinformatics analysisoral squamous cell carcinoma

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