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miRNA-200b Signature in the Prevention of Skin Cancer Stem Cells by Polyphenol-enriched Blueberry Preparation

Nawal Alsadi1, Jean-François Mallet1, Chantal Matar1,2

  • 1Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Canada.

Journal of Cancer Prevention
|October 27, 2021
PubMed

Insights

Polyphenol-enriched blueberry preparation (PEBP) effectively inhibited skin cancer stem cell proliferation and reduced metastasis. PEBP also upregulated tumor-suppressing microRNAs, demonstrating its potential as a natural skin cancer chemopreventive agent.

Area of Science:

  • * Dermatology and Oncology
  • * Natural Product Chemistry
  • * Epigenetics and Molecular Biology

Background:

  • * Solar UV radiation exposure is a primary cause of skin cancer, inducing DNA damage and cellular pathway dysregulation.
  • * Photochemoprevention using natural products, particularly polyphenols, offers a promising strategy for controlling skin cancer.
  • * Polyphenols can inhibit cancer stem cells (CSCs) and modulate microRNA expression, impacting cancer progression.

Purpose of the Study:

  • * To evaluate the effects of polyphenol-enriched blueberry preparation (PEBP) and non-fermented blueberry juice (NBJ) on microRNA expression and target proteins in skin cancer.
  • * To assess the impact of PEBP and NBJ on skin CSC stemness, motility, and invasiveness.

Main Methods:

  • * Treatment of melanoma cell lines (HS 294T and B16F10) with PEBP and NBJ.
  • * Analysis of skin CSC proliferation, melanophore formation, and CD133+ stem cell marker expression.
  • * Quantification of microRNA (miR-200s) and target protein (ZEB1) expression levels.

Main Results:

  • * PEBP significantly inhibited the proliferation of skin CSCs and reduced melanophore formation in melanoma cell lines.
  • * PEBP treatment led to a significant decrease in CD133+ stem cell marker expression compared to NBJ and control groups.
  • * PEBP upregulated tumor suppressors miR-200s and modulated their target protein ZEB1, indicating anti-metastatic effects.

Conclusions:

  • * PEBP exhibits significant anticancer and anti-metastatic properties against skin cancer.
  • * The study highlights PEBP's potential as a novel chemopreventive agent for skin cancer.
  • * Modulation of microRNA expression and CSC markers by PEBP provides mechanistic insights into its therapeutic effects.
Keywords:
Cancer stem cellsMicroRNA-200bNeoplasm metastasisPolyphenolsZinc finger E-box binding homeobox 1

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