Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Phragmoplast

The Phragmoplast

Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Two-step, long-distance nuclear migration guided by distinct actin networks prior to plant root hair formation.

PNAS nexus·2026

Same author

The immunity-associated protein ACIP1 counteracts MIDD1 to coordinate xylem cell wall patterning.

Current biology : CB·2026

Same author

The conserved machinery of bipolar prospindle formation controls acentrosomal spindle orientation in land plants.

Current biology : CB·2025

Same author

Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.

Nature communications·2025

Same author

Structural analysis of microtubule binding by minus-end targeting protein Spiral2.

Biochimica et biophysica acta. Molecular cell research·2024

Same author

Guidelines for naming and studying plasma membrane domains in plants.

Nature plants·2024

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 15, 2025

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

A Quantitative Method for Evaluating Phragmoplast Morphology.

Takema Sasaki^1,2, Yoshihisa Oda^3,4

¹Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Shizuoka, Japan. stakema@nig.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2021

Summary

This summary is machine-generated.

Quantifying phragmoplast width is crucial for studying plant cell division. This study presents a new method to measure phragmoplast dimensions, aiding research on microtubule-associated proteins and their role in cell plate formation.

Keywords:

Cytokinesis Imaging analysis Microtubules Phragmoplast

More Related Videos

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

Related Experiment Videos

Last Updated: Oct 15, 2025

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Published on: November 27, 2014

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

Area of Science:

Cell Biology
Plant Biology
Cytoskeleton Dynamics

Background:

Phragmoplasts are essential plant-specific microtubule structures.
They form the cell plate during cytokinesis, guiding cell division.
Mutations in microtubule-associated proteins disrupt phragmoplast morphology.

Purpose of the Study:

To develop and present a method for quantifying phragmoplast width.
To enable accurate assessment of phragmoplast morphology in plant mutants.
To facilitate research on the function of microtubule-associated proteins in cell division.

Main Methods:

Description of a novel method for phragmoplast width quantification.
Application of the method to assess mutant phenotypes.
Image analysis techniques for measuring microtubule structures.

Main Results:

A reliable method for quantifying phragmoplast width has been established.
This method allows for precise measurement of phragmoplast dimensions.
The quantification aids in understanding the impact of mutations on phragmoplast morphology.

Conclusions:

Accurate quantification of phragmoplast width is vital for plant cell biology research.
The presented method provides a valuable tool for analyzing phragmoplast defects.
This facilitates the study of microtubule-associated proteins and cell plate formation.