Related Experiment Video
Updated: Oct 15, 2025
09:18
Simple Methods for the Preparation of Non-noble Metal Bulk-electrodes for Electrocatalytic Applications
Published on: June 21, 2017
11.6K
Bulk Electrocatalytic NADH Cofactor Regeneration with Bipolar Electrochemistry
Chunhua Zhang1, Huiting Zhang1, Junying Pi1
1Engineering Research Center for Nanomaterials, Henan University, Kaifeng, China.
Angewandte Chemie (International Ed. in English)
|October 27, 2021
Summary
Electrochemical regeneration of reduced nicotinamide adenine dinucleotide (NADH) is crucial for electroenzymatic synthesis. A novel bipolar electrochemical cell using dispersed microelectrodes offers efficient, wireless NADH regeneration, overcoming scale-up limitations.
Area of Science:
- Electrochemistry
- Bioelectrochemistry
- Catalysis
Background:
- Electrochemical regeneration of reduced nicotinamide adenine dinucleotide (NADH) is vital for synthesizing valuable chemicals via electroenzymatic routes.
- Scale-up of current methods is hindered by mass transport limitations associated with large electrodes.
Purpose of the Study:
- To develop a novel approach for efficient electrochemical NADH regeneration.
- To overcome scale-up challenges in electroenzymatic synthesis using a bipolar electrochemical cell.
Main Methods:
- Utilizing a dispersion of electrocatalytically active modified microparticles within a bipolar electrochemical cell.
- Employing [Rh(Cp*)(bpy)Cl]+ functionalized carbon microbeads as bipolar objects.
- Investigating the electroregeneration of NADH at the negatively polarized face of the microparticles.
Main Results:
- Demonstrated successful electroenzymatic synthesis using dispersed microelectrodes.
- Achieved wireless NADH regeneration without direct electrical connection to individual microelectrodes.
- Showcased that system efficiency is tunable by electric field strength and microelectrode concentration.
Conclusions:
- A wireless bioelectrocatalytic approach using dispersed microelectrodes in a bipolar cell is effective for NADH regeneration.
- This method overcomes mass transport limitations, offering a scalable solution for electroenzymatic synthesis.
- The proposed system presents promising perspectives for bulk-phase electroenzymatic synthesis.
Related Concept Videos
02:47Voltaic/Galvanic Cells
59.2K
Spontaneous Chemical Reactions
Spontaneous redox reactions occur abundantly in nature. The chemical reaction occurring in a disposable AA battery powering our remote controls is one such example of a spontaneous redox reaction. Another example is the immersion of coiled copper wire into an aqueous silver nitrate solution. The reaction shows a gradual, visually impressive color change from colorless to bright blue and the formation of a grey precipitate on the copper wire. In this experiment,...
Spontaneous redox reactions occur abundantly in nature. The chemical reaction occurring in a disposable AA battery powering our remote controls is one such example of a spontaneous redox reaction. Another example is the immersion of coiled copper wire into an aqueous silver nitrate solution. The reaction shows a gradual, visually impressive color change from colorless to bright blue and the formation of a grey precipitate on the copper wire. In this experiment,...
59.2K
03:00Electrolysis
27.9K
In a galvanic cell, the electrical work is done by a redox system on its surroundings as electrons produced by the spontaneous redox reactions are transferred through an external circuit. Alternatively, an external circuit does work on a redox system by imposing a voltage sufficient to drive an otherwise nonspontaneous reaction in a process known as electrolysis. For instance, recharging a battery involves the use of an external power source to drive the spontaneous (discharge) cell reaction in...
27.9K
01:26Thermal and Photochemical Electrocyclic Reactions: Overview
2.5K
Electrocyclic reactions are reversible reactions. They involve an intramolecular cyclization or ring-opening of a conjugated polyene. Shown below are two examples of electrocyclic reactions. In the first reaction, the formation of the cyclic product is favored. In contrast, in the second reaction, ring-opening is favored due to the high ring strain associated with cyclobutene formation.
2.5K
01:29Role of Reduced Coenzymes NADH and FADH₂
14.0K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
14.0K
01:10Voltammetric Techniques: Cyclic Voltammetry
795
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
795
03:12Batteries and Fuel Cells
28.6K
A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
28.6K