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Human recipient cell for oncogene transfection studies
Abstract:
We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.
Insights
Human osteosarcoma cells were successfully transformed into tumor-forming cells using oncogene DNA. These HOS cells offer a valuable human cell model for oncogene transfection studies, replacing mouse cells.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Osteosarcoma is a significant bone cancer.
- Developing reliable cell models for cancer research is crucial.
- Previous studies often relied on murine (mouse) cell lines.
Purpose of the Study:
- To transform a non-tumorigenic human osteosarcoma cell line (HOS TE-85 clone 5) into a tumorigenic one.
- To establish a human cell-based system for oncogene transfection studies.
- To evaluate HOS cells as an alternative to NIH 3T3 cells.
Main Methods:
- Transfection of HOS TE-85 clone 5 cells with human oncogene DNA.
- Introduction of genetic markers via genomic DNA samples.
- Tumorigenicity assays in athymic nude mice.
Main Results:
- Successful transformation of HOS cells to tumorigenicity.
- Short latency periods (as short as 3 weeks) observed in mice.
- Demonstrated transformation using genetic markers in genomic DNA.
Conclusions:
- HOS cells can be reliably transformed to tumorigenicity using oncogene DNA.
- HOS cells serve as a practical human cell alternative to NIH 3T3 cells for oncogene research.
- This model facilitates studies on human osteosarcoma development and oncogene function.