Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of A-DNA in solution.

E Charney, H H Chen

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    DNA in trifluoroethanol solutions adopts the A-conformation, confirmed by electric linear dichroism. Both DNA and RNA fragments show A-form structures in solution, matching crystalline states.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Analysis of risk factors for early death in NPM1-mutated acute myeloid leukemia patients].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2026
    Same author

    [Construction and validation of clinical prediction model of tongue base collapse under drug-induced sleep endoscopy in OSA patients].

    Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2024
    Same author

    [Immune reconstitution and influencing factors in HIV infected men who have sex with men with access to antiviral therapy in Guangxi Zhuang Autonomous Region from 2005 to 2021].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
    Same author

    Evaluation of factors associated with high advanced HIV disease and mortality in Southwestern China: a retrospective cohort study, 2005-2020.

    Public health·2024
    Same author

    [Analysis of HIV transmission hotspots and characteristics of cross-regional transmission in Guangxi Zhuang Autonomous Region based on molecular network].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2022
    Same author

    [Factors associated with death and attrition in HIV-infected children under initial antiretroviral therapy in Guangxi Zhuang Autonomous Region, 2004 - 2019].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2022
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Area of Science:

    • Biophysics
    • Molecular Biology
    • Structural Biology

    Background:

    • DNA typically exists in the B-conformation in aqueous solutions.
    • The A-conformation of DNA is often observed in dehydrated conditions or in the presence of certain organic solvents.
    • Understanding DNA structure in different environments is crucial for molecular biology.

    Purpose of the Study:

    • To investigate the structural conformation of DNA and RNA fragments in aqueous trifluoroethanol solutions.
    • To determine if DNA and RNA adopt the A-conformation in these solutions.
    • To compare structural parameters of DNA in solution with crystalline forms.

    Main Methods:

    • Electric linear dichroism (ELD) was used to study DNA and RNA structure.
    • Measurements were performed on phi X174 DNA (5386 bp) and a 172-bp sea urchin 5S RNA gene fragment.

    Related Experiment Videos

  • ELD data analysis involved determining reduced dichroism and base-pair translation from relaxation times.

    • Main Results:

    • Both phi X174 DNA and the 172-bp RNA fragment adopted the double-helical A-form in >70% trifluoroethanol solutions.
    • Key structural parameters like base tilt and base-pair translation in solution matched those of crystalline A-form DNA.
    • The reduced dichroism of A-DNA in solution was independent of molecular length (172-5386 bp), unlike B-DNA.

    Conclusions:

    • DNA and RNA exist in the A-conformation in concentrated trifluoroethanol solutions.
    • The A-form structure in solution is highly similar to the solid-state A-form, with conserved base tilt and translation.
    • Molecular length affects B-DNA dichroism in solution, but not A-DNA dichroism in alcoholic solutions.