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Updated: Oct 15, 2025

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Published on: June 13, 2022
Diaminomaleonitrile derivatives as new potential antichagasic compounds: a study of structure-activity relationships
Aldo S de Oliveira1,2, Lucas Dos S Mello3, Camila H Ogihara4
1Federal University of Santa Catarina, Campus of Blumenau, Department of Exact Sciences & Education, Rua João Pessoa, 2750, Velha, Blumenau - SC, 89036-256, Brazil.
Abstract:
Background: Schiff bases are synthetically accessible compounds that have been used in medicinal chemistry. Methods & results: In this work, 27 Schiff bases derived from diaminomaleonitrile were synthesized in high yields (80-98%). Molecular docking studies suggested that the Schiff bases interact with the catalytic site of cruzain. The most active cruzain inhibitor, analog 13 (IC50 = 263 nM), was predicted to form an additional hydrophobic contact with Met68 in the binding site of the enzyme. A strong correlation between the IC50 values and ChemScore binding energies was observed (R = 0.99). Kernel-based 2D quantitative structure-activity relationship models for the whole dataset yielded sound correlation coefficients (R2 = 0.844; Q2 = 0.719). Conclusion: These novel and potent cruzain inhibitors are worthwhile starting points in further Chagas disease drug discovery programs.
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