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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of Pseudognaphalium affine (D.Don) Anderb. (Asteraceae)
Li Xie1, Jiale Zhao2, Rong Liu1
1School of Preclinical Medicine, Chengdu University, Chengdu, China.
Abstract:
Pseudognaphalium affine (D.Don) Anderb. is an annual herbaceous plant used as a vegetable and traditional medicine. Here, we sequenced and assembled the complete chloroplast genome of P. affine. The plastome is 151,573 bp in size with a pair of inverted repeat regions (IRs) of 24,849 bp each, a large single-copy region (LSC) of 83,632 bp, and a small single-copy region (SSC) of 18,243 bp. The overall GC content of the whole plastome is 37.33%, and the IR regions are more GC rich (43.08%) than the LSC (35.30%) and SSC (31.03%) regions. It contains 129 genes, including 83 protein-coding genes, 36 tRNAs, eight rRNAs, and two pseudogenes. Phylogenetic analysis showed that P. affine is most closely related to Leontopodium leiolepis. This genome will provide a useful genetic resource for future conservation, evolution, and phylogeny studies of P. affine and the tribe Inuleae.
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